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ApexBio arp100 mmp2 inhibitor

(A) DU145 cells with filamin C silencing were fluorescence-labeled and analyzed in a zebrafish cancer metastasis model. The selected pictures were shown from 0 to 3 days post injection. The areas indicated with arrows were enlarged for visualizing disseminated cancer cells. (B) Filamin C was silenced in DU145 cells and the proenzyme (Pro) and activated form of <t>MMP2</t> were analyzed. (C) Migration of DU145 cells with or without filamin C silencing were analyzed. The third group with filamin C silencing was further treated with the MMP2 inhibitor <t>ARP100.</t> The statistical analysis results were shown in Figure and the Western blot results were shown in Figure . FLNC, filamin C.

Arp100 Mmp2 Inhibitor, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arp100+mmp2+inhibitor/arp+100++specific+mmp+2+inhibitor/pmc04359225-292-17-20
Average 90 stars, based on 1 article reviews

arp100 mmp2 inhibitor - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells"

Article Title: Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells

Journal: Oncotarget

doi:

Figure Legend Snippet: (A) DU145 cells with filamin C silencing were fluorescence-labeled and analyzed in a zebrafish cancer metastasis model. The selected pictures were shown from 0 to 3 days post injection. The areas indicated with arrows were enlarged for visualizing disseminated cancer cells. (B) Filamin C was silenced in DU145 cells and the proenzyme (Pro) and activated form of MMP2 were analyzed. (C) Migration of DU145 cells with or without filamin C silencing were analyzed. The third group with filamin C silencing was further treated with the MMP2 inhibitor ARP100. The statistical analysis results were shown in Figure and the Western blot results were shown in Figure . FLNC, filamin C.

Techniques Used: Fluorescence, Labeling, Injection, Migration, Western Blot

Cell Culture:

Article Title: Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells
Article Snippet: .. After cultured for 36 h, the cells transfected with filamin C siRNA were treated with 50 μM ARP100 MMP2 inhibitor (Apexbio, Shanghai, China) and cultured for 12 h before the evaluation of inhibitory effects. ..

Transfection:

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Correlation between MMP2 expression and clinicopathological features of CRC patients T: tumor depth; N: lymph node involvement; M: metastasis; MMP2: matrix metalloproteinase-2; CRC: colorectal cancer * Based on Chi-square test, or (if not applicable) Fisher’s exact test

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: Correlation between MMP2 expression and clinicopathological features of CRC patients T: tumor depth; N: lymph node involvement; M: metastasis; MMP2: matrix metalloproteinase-2; CRC: colorectal cancer * Based on Chi-square test, or (if not applicable) Fisher’s exact test

Article Snippet: Subsequently, the dishes were further incubated with 5 μM of the MMP2 inhibitor ARP100 (MMP-2 inhibitor from Santa Cruz Biotechnology, Dallas, USA), dissolved in dimethyl sulfoxide (DMSO) at the specified concentration as needed for the experiments.

Techniques: Expressing

Differences in MMP2 expression between cancerous and cancerous-adjacent normal tissues * Based on Chi-square test, or (if not applicable) Fisher’s exact test; MMP2: matrix metalloproteinase-2

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: Differences in MMP2 expression between cancerous and cancerous-adjacent normal tissues * Based on Chi-square test, or (if not applicable) Fisher’s exact test; MMP2: matrix metalloproteinase-2

Techniques: Expressing

The representative image showed elevated MMP2 expression levels in (A) cancerous tissues compared to those in (B) normal tissues (20x). MMP2: matrix metalloproteinase-2

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: The representative image showed elevated MMP2 expression levels in (A) cancerous tissues compared to those in (B) normal tissues (20x). MMP2: matrix metalloproteinase-2

Techniques: Expressing

MMP2 was inhibited in SW480 cells by treating with 5 μM of the MMP2 inhibitor ARP100 for 24h, while control cells were treated with DMSO. (A-C) Transwell migration and invasion assays (magnification: 100×) demonstrated that MMP2 inhibition significantly reduced the migratory and invasive capabilities of SW480 cells. (D and E) Wound healing assays (scale: 100 µm) showed that MMP2 inhibition markedly decreased gap closure in SW480 cells. Data are presented as mean ± standard error (**P≤0.002; ***P<0.0005). (F) Western blot analysis confirmed MMP2 inhibition. Results are representative of three independent experiments. MMP2: matrix metalloproteinase-2; CRC: colorectal cancer; DMSO: dimethyl sulfoxide

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: MMP2 was inhibited in SW480 cells by treating with 5 μM of the MMP2 inhibitor ARP100 for 24h, while control cells were treated with DMSO. (A-C) Transwell migration and invasion assays (magnification: 100×) demonstrated that MMP2 inhibition significantly reduced the migratory and invasive capabilities of SW480 cells. (D and E) Wound healing assays (scale: 100 µm) showed that MMP2 inhibition markedly decreased gap closure in SW480 cells. Data are presented as mean ± standard error (**P≤0.002; ***P<0.0005). (F) Western blot analysis confirmed MMP2 inhibition. Results are representative of three independent experiments. MMP2: matrix metalloproteinase-2; CRC: colorectal cancer; DMSO: dimethyl sulfoxide

Techniques: Control, Migration, Inhibition, Western Blot

MMP2 was inhibited in SW480 cells by treating with 5 μM of the MMP2 inhibitor ARP100, while control cells were treated with DMSO. Cell proliferation was assessed using the MTT assay, with absorbance readings taken at 570 nm. (A) APR100 inhibitor significantly inhibited SW480 cell proliferation. (B and C) Transfection efficiency was validated through RT-PCR and western blot analysis. Data are presented as mean ± standard error (*P<0.02 and **P≤0.009). Results from three representative experiments are depicted. MMP2: matrix metalloproteinase-2; DMSO: dimethyl sulfoxide; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide; RT-PCR: reverse transcription-polymerase chain reaction

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: MMP2 was inhibited in SW480 cells by treating with 5 μM of the MMP2 inhibitor ARP100, while control cells were treated with DMSO. Cell proliferation was assessed using the MTT assay, with absorbance readings taken at 570 nm. (A) APR100 inhibitor significantly inhibited SW480 cell proliferation. (B and C) Transfection efficiency was validated through RT-PCR and western blot analysis. Data are presented as mean ± standard error (*P<0.02 and **P≤0.009). Results from three representative experiments are depicted. MMP2: matrix metalloproteinase-2; DMSO: dimethyl sulfoxide; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide; RT-PCR: reverse transcription-polymerase chain reaction

Techniques: Control, MTT Assay, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Reverse Transcription, Polymerase Chain Reaction

Caspase 3 and 9 activities in SW480 treated cells were quantified using spectrophotometry. The activity of caspases in control cells (treated with DMSO) was set as 1, and the activity values of the experimental group were standardized relative to this. (A) SW480 cells treated with inhibitor exhibited a significant elevation in both caspase 3 and caspase 9 activities. (B) Western blot analysis was performed to detect the expression levels of apoptosis-related proteins. Bar graphs depict the standard errors of the mean from three experiments (**P≤0.005 or P=0.001). MMP2: matrix metalloproteinase-2; DMSO: dimethyl sulfoxide

Journal: Cureus

Article Title: The Role of Matrix Metalloproteinase-2 (MMP2) in Colorectal Cancer Progression: Correlation With Clinicopathological Features and Impact on Cellular Processes

doi: 10.7759/cureus.61941

Figure Lengend Snippet: Caspase 3 and 9 activities in SW480 treated cells were quantified using spectrophotometry. The activity of caspases in control cells (treated with DMSO) was set as 1, and the activity values of the experimental group were standardized relative to this. (A) SW480 cells treated with inhibitor exhibited a significant elevation in both caspase 3 and caspase 9 activities. (B) Western blot analysis was performed to detect the expression levels of apoptosis-related proteins. Bar graphs depict the standard errors of the mean from three experiments (**P≤0.005 or P=0.001). MMP2: matrix metalloproteinase-2; DMSO: dimethyl sulfoxide

Techniques: Spectrophotometry, Activity Assay, Control, Western Blot, Expressing

MMP9 expression and activity and EMT markers change in AGS and HGC27 sublines. ( A ) RT-PCR showed the expression of MMP1, 2, 3 and 9 was increased in HGC27 WISP-2 kd cells and there was no obvious difference of MMP7 compared with controls. Expression of MMP7 was downregulated in AGS WISP-2 kd cells, MMP1, 2, 3 and 9 were upregulated, however, there was no obvious expression of MMP2 in AGS WISP-2 kd cells compared with controls. ( B ) Gelatine zymography indicated the reduced emzyme activity of MMP9 in AGS WISP-2 kd cells treated with ERK and JNK inhibitors and reduced MMP9/2 in HGC27 WISP-2 kd cells treated with JNK inhibitors. 1: pro-MMP9, 2: MMP9 and 3: MMP2. ( C ) Knockdown of WISP-2 caused a significant increase in the invasiveness of AGS WISP-2 kd cells treated with MMP9 inhibitor (Marimastat) compared with the AGS WISP-2 kd cells without treatment (* P <0.05). ( D ) Representative images of cells following staining: after 72 h' incubation on an artificial Matrigel basement membrane, the difference of the numbers of invasive cells was significantly observed in groups treated with MMP9 inhibitor compared with the control group. ( E and F ) EMT marker expression differences after WISP-2 knockdown in AGS and HGC27 cells. Expression levels of Twist, N-cadherin and Vimentin were increased and E-cadherin were decreased. And there was no difference in Slug expression.

Journal: British Journal of Cancer

Article Title: WISP-2 in human gastric cancer and its potential metastatic suppressor role in gastric cancer cells mediated by JNK and PLC- γ pathways

doi: 10.1038/bjc.2015.285

Figure Lengend Snippet: MMP9 expression and activity and EMT markers change in AGS and HGC27 sublines. ( A ) RT-PCR showed the expression of MMP1, 2, 3 and 9 was increased in HGC27 WISP-2 kd cells and there was no obvious difference of MMP7 compared with controls. Expression of MMP7 was downregulated in AGS WISP-2 kd cells, MMP1, 2, 3 and 9 were upregulated, however, there was no obvious expression of MMP2 in AGS WISP-2 kd cells compared with controls. ( B ) Gelatine zymography indicated the reduced emzyme activity of MMP9 in AGS WISP-2 kd cells treated with ERK and JNK inhibitors and reduced MMP9/2 in HGC27 WISP-2 kd cells treated with JNK inhibitors. 1: pro-MMP9, 2: MMP9 and 3: MMP2. ( C ) Knockdown of WISP-2 caused a significant increase in the invasiveness of AGS WISP-2 kd cells treated with MMP9 inhibitor (Marimastat) compared with the AGS WISP-2 kd cells without treatment (* P <0.05). ( D ) Representative images of cells following staining: after 72 h' incubation on an artificial Matrigel basement membrane, the difference of the numbers of invasive cells was significantly observed in groups treated with MMP9 inhibitor compared with the control group. ( E and F ) EMT marker expression differences after WISP-2 knockdown in AGS and HGC27 cells. Expression levels of Twist, N-cadherin and Vimentin were increased and E-cadherin were decreased. And there was no difference in Slug expression.

Article Snippet: The MMP9 inhibitor (Marimastat, Cat number: 2631) and MMP2 inhibitor (ARP100, Cat number: 2621) were both from Tocris Bioscience; Matrigel was from BD Bio-Science (Oxford, UK; Cat Number: 354234).

Techniques: Expressing, Activity Assay, Reverse Transcription Polymerase Chain Reaction, Zymography, Knockdown, Staining, Incubation, Membrane, Control, Marker

Journal: Oncotarget

Article Title: Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells

doi:

Figure Lengend Snippet: (A) DU145 cells with filamin C silencing were fluorescence-labeled and analyzed in a zebrafish cancer metastasis model. The selected pictures were shown from 0 to 3 days post injection. The areas indicated with arrows were enlarged for visualizing disseminated cancer cells. (B) Filamin C was silenced in DU145 cells and the proenzyme (Pro) and activated form of MMP2 were analyzed. (C) Migration of DU145 cells with or without filamin C silencing were analyzed. The third group with filamin C silencing was further treated with the MMP2 inhibitor ARP100. The statistical analysis results were shown in Figure and the Western blot results were shown in Figure . FLNC, filamin C.

Article Snippet: After cultured for 36 h, the cells transfected with filamin C siRNA were treated with 50 μM ARP100 MMP2 inhibitor (Apexbio, Shanghai, China) and cultured for 12 h before the evaluation of inhibitory effects.

Techniques: Fluorescence, Labeling, Injection, Migration, Western Blot

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arp100 mmp2 inhibitor (ApexBio)

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1) Product Images from "Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells"

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